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The cystine ligand was clearly resolved in the electron density map and sits at the base of the polar pocket observed in the apo state (Fig.2a, b). Cystine adopts an extended conformation inside the binding web page and tends to make many notable interactions to conserved side chains (Fig.2c, d). Cystine can exist in three various ionic forms based on the pH of the environment38. Inside physiological pH the carboxyl groups will be totally dissociated, on the other hand, the two amino groups have distinct pKa values of 7.48 and 9.02. Our crystallisation trials had been performed at pH 6.5,...