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Two more classes of EM maps were also deduced from the cryo-EM information, which corresponded to the D0UDD and D0DDD arrangements, corresponding to 7%, and 11% of the total population, respectively (Supplementary Fig.14). The T326I variant also exhibited a D0UUU arrangement whose EM map could fit to the IPEC-J2 cell-derived PEDV PT52 S atomic model, but the relative population was a lot reduced (23%). Alternatively, the T326I variant populated much more D0-down conformation with the 19%, 16% and 42% of the population getting in the D0UUD, D0UDD and D0DDD arrangements, respectively (Supplementary Fig.14). Quantification of the relative D0-up vs D0-down...